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Home Species Homo sapiens
IPS-derived Cell Model

Basic Information

Homo sapiens / IPS-derived Cell Model
Species: Human
Category: Human Biological Model
Description: The iPS-derived and differentiated cells allow researchers to study the impact of a distinct cell type in health and disease as well as performing therapeutic drug screens on a human genetic background. The study of IPS-derived Cell model may enable the exploration of mechanisms underlying human disease and human development, and provide a platform for screening the efficacy and toxicity of pharmaceuticals.

Gene Summary

mRNA

ICG00153-81
ACTB C1orf43 PSMB4 GAPDH HMBS
ICG00153-82
RPLP0 UBC CAPN10

Studies (2)

ICG00153-81

Ips-Derived Cell Model
Neural Stem Cell
Human Biological Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
ACTB Actin
Ipsc
Neural Stem Cell
Derived Neurons
 NM_001101
F: CTGGAACGGTGAAGGTGACA
R: AAGGGACTTCCTGTAACAATGCA
 - 60 SYBR
C1orf43 Chromosome 1 Open Reading Frame
Ipsc
Neural Stem Cell
Derived Neurons
 -
F: ACGCCTTTCAAGGGTGTACG
R: CAAAGACCCCTGTCCCATAGC
 - 60 SYBR
PSMB4 Proteasome
Ipsc
Neural Stem Cell
Derived Neurons
 -
F: CATTCCGTCCACTCCCGATT
R: CGAACTTAACGCCGAGGACT
 - 60 SYBR
GAPDH Glyceraldehyde-3-Phosphate Dehydrogenase
Ipsc
Neural Stem Cell
Derived Neurons
 NM_002046
F: TGCACCACCAACTGCTTAGC
R: GGCATGGACTGTGGTCATGAG
 - 60 SYBR
HMBS Hydroxymethylbilane Synthase
Ipsc
Neural Stem Cell
Derived Neurons
 NM_000190
F: GGCAATGCGGCTGCAA
R: GGGTACCCACGCGAATCAC
 - 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder

Contact

Name: A.S. Artyukhov
Email: aleksandr.artyukhov@phystech.edu
Institution: Moscow Institute of Physics and Technology, Dolgoprudny, Russia

Reference

New genes for accurate normalization of qRT-PCR results in study of iPS and iPS-derived cells.
Artyukhov AS, Dashinimaev EB, Tsvetkov VO, Bolshakov AP, Konovalova EV, Kolbaev SN, Vorotelyak EA, Vasiliev AV.
Gene. 2017-05-22

ICG00153-82

Ips-Derived Cell Model
Human Biological Model

Internal Control Genes

Gene Symbol Gene Model Application Scenario Accession Number Primers(5' - 3')
(Forward/Reverse)		 Size (bp) Annealing Temperature Detection
RPLP0 Ribosomal Protein LP
Neural Stem Cell
Induced Pluripotent Stem Cell
 NM_001002.3
F: CCTCGTGGAAGTGACATCGT
R: CTGTCTTCCCTGGGCATCAC
 76 60 SYBR
UBC Ubiquitin
Early Neural Progenitor
Induced Pluripotent Stem Cell
 NM_021009.6
F: ACGGGACTTGGGTGACTCTA
R: ATCGCCGAGAAGGGACTACT
 82 60 SYBR
CAPN10 Calpain
Neural Progenitor
Induced Pluripotent Stem Cell
 NM_023083.3
F: TCTCACCGGGCTACTACCTG
R: CCCGGTAGAGAAGACTCGGA
 86 60 SYBR

Molecular Types:

mRNA

Evaluation Methods

geNorm
NormFinder
BestKeeper

Contact

Name: Leonora Buzanska
Email: buzanska@imdik.pan.pl
Institution: Department of Stem Cell Bioengineering, Mossakowski Medical Research Centre Polish Academy of Sciences, 5 Pawinskiego Str., 02-106 Warsaw, Poland

Reference

Reference Gene Validation via RT-qPCR for Human iPSC-Derived Neural Stem Cells and Neural Progenitors.
Augustyniak J, Lenart J, Lipka G, Stepien PP, Buzanska L.
Molecular neurobiology. 2019-03-29